Antileishmanial activity of nanoliposomes  containing curcumin in vitro and in vivo

Akbari, Morteza; Askari Zadeh, Anis; Sadri, Kayvan; Iranshahi, Mehrdad; Jaafari, Mahmoud Reza; Khamesipour, Ali; Badiee, Ali

Volume 8, Issue 4 (Volume 8, Number 4 2018) jdc 2018, 8(4): 204-217 | Back to browse issues page

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Akbari M, Askari Zadeh A, Sadri K, Iranshahi M, Jaafari M R, Khamesipour A et al . Antileishmanial activity of nanoliposomes containing curcumin in vitro and in vivo . jdc 2018; 8 (4) :204-217
URL: http://jdc.tums.ac.ir/article-1-5283-en.html

Antileishmanial activity of nanoliposomes containing curcumin in vitro and in vivo

Morteza Akbari¹

, Anis Askari Zadeh¹

, Kayvan Sadri²

, Mehrdad Iranshahi³

, Mahmoud Reza Jaafari⁴

, Ali Khamesipour⁵

, Ali Badiee ^*

⁶

1- Novin Pharmaceutical, Nanotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
2- Nuclear Medicine Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
3- Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
4- Nanotechnology Research Center, Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
5- Center for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, Iran
6- Novin Pharmaceutical, Nanotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran , badieea@mums.ac.ir

Abstract: (4357 Views)

Background and Aim: Curcumin possesses diverse pharmacologic effects including anti-inflammatory, antioxidant, antiproliferative and antiangiogenic activities. The aim of this study was to develop liposomal formulation of curcumin and evaluate the leishmanial effects of its topical use against L. major in vitro and in vivo.

Methods: Nanoliposomes containing 1, 2 or 3% curumin were prepared by fusion method and characterized for their size, morphology and stability. The in vitro efficacy of liposomal form of Curcumin against promastigotes and amastigotes form of L. major was also evaluated. Moreover, the leishmanial effect of formulations was evaluated in infected BALB/c mice. The lesions were treated topically by different formulations twice a day, for 4 weeks. During this time and 4 weeks after treatment, lesions size was measured every week. The level of splenic parasite burdens was investigated in week 4 after parasite inoculation.

Results: Curcumin showed no leishmanicidal effect against live promasigotes and amastigotes in culture media. There was no significant difference between treated and control animals regarding lesion size and splenic parasite burden.

Conclusion: The results of this study showed that nanoliposomes containing curcumin in topical form has no significant leishmanicidal effect in vitro and in vivo against
L. major.

Keywords: curcumin, leishmaniasis, L. major, liposome

Full-Text [PDF 1327 kb] (1301 Downloads)

Type of Study: Research | Subject: Special
Received: 2018/03/4 | Accepted: 2018/03/4 | Published: 2018/03/4