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Background and aim: Human dermal fibroblasts (HDFs) are key regulators of skin homeostasis through extracellular matrix remodeling. Polydeoxyribonucleotides (PDRN) and magnesium ascorbyl phosphate (MAP) are recognized as skin regenerative agents, but their molecular effects on HDFs are not fully elucidated. This study aimed to investigate the effects of PDRN and MAP on cellular proliferation and type I collagen gene expression in HDFs.
 

Methods: The HDF cell line was treated with PDRN and MAP, and cell viability was evaluated using the MTT assay at 24, 48, and 72 hours. Based on viability outcomes, optimal concentrations of PDRN (100 ng/ml) and MAP (250 µg/ml) were selected. Seventy-two hours post-treatment, the expression levels of KI-67 and COL1A1 genes were analyzed via qPCR (P<0.05).
 

Results: Cell viability analyses demonstrated significant time- and dose-dependent responses for both PDRN and MAP. At the molecular level, independent treatments with PDRN and MAP upregulated KI-67 gene expression, confirming an enhanced cellular proliferative capacity. Conversely, in a divergent manner, COL1A1 gene expression was downregulated under these exact treatment conditions.
 

Conclusion: PDRN and MAP induce a divergent response in HDFs; while promoting mitogenic activity, they simultaneously suppress fibrogenesis and COL1A1 expression. This cellular reprogramming underscores the therapeutic potential of these compounds in anti-fibrotic strategies and scarless wound healing. However, in vivo studies are required to conclusively validate these findings.